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目的：检测白细胞介素-8（IL－8）样免疫阳性物质在大鼠垂体前叶中的存在和分布．方法：用抗入IL－8多克隆抗体行ABC免疫组织化学染色．结果：正常大鼠垂体前叶存在IL－8样免疫阳性物质；阳性细胞主要位于大鼠垂体前叶的浅层区域；大鼠出生3d内已有免疫阳性物质存在，在15d左右形成其分布规律；IL－8样免疫阳性物质主要存在于GH细胞．结论：正常大鼠垂体前叶稳定性存在IL－8样免疫阳性物质，提示IL－8可能影响垂体前叶细胞增殖和（或）激素分泌，从而在神经内分泌免疫网络中发挥一定的作用。
Objective: To detect the presence and distribution of interleukin-8 (IL-8) -like immunopositive substances in rat anterior pituitary. Methods: ABC immunohistochemical staining was performed using anti-IL-8 polyclonal antibody. Results: There were IL-8-like immunoreactive substances in the anterior pituitary of normal rats. The positive cells mainly located in the superficial area of the anterior pituitary in rats. The immunopositive substances existed within 3 days after birth and formed in 15 days ; IL-8-like immunopositive substances are mainly found in GH cells. CONCLUSION: IL-8-like immunopositive substances exist in the anterior pituitary of normal rats, suggesting that IL-8 may affect the proliferation and / or hormone secretion of anterior pituitary cells and play a role in the neuroendocrine immune network.