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为了分析和克隆葡萄球菌肠毒素(SE)A全长基因,设计了一对针对SEA全长基因的特异性引物,成功地用PCR反应从标准产SEA的葡萄球菌基因组中扩增出了一条约770bp的条带,为进一步用PCR法克隆SEA基因,并把它用于抗肿瘤研究奠定了实验基础
To analyze and clone the full-length staphylococcal enterotoxin (SE) gene, a pair of specific primers specific for the full-length SEA gene was designed and a protocol was successfully amplified from the Staphylococcus aureus genome of the standard SEA by PCR 770bp band, in order to further clone the SEA gene by PCR, and use it for anti-tumor research laid the foundation for the experiment