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纯系新西兰大白兔经角膜接种ＨＳＶ－ｌｓｔｏｋｅｋ株，成功建立单疱病毒性角膜炎模型。自愈后４５天，以原病变角膜为供体，健康新西兰白兔为受体，行穿通性角膜移植术（ＰＫＰ）同时，取患侧１０份及健侧１０份三叉神经节，应用多聚酶链反应（ＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ，ＰＣＲ）检测ＨＳＶ－１ＤＮＡ。术后５０天，取术侧角膜植片１０只及术侧三叉神经节１０份作相同检测。结果表明，患侧三叉神经节１０份中８份ＨＳＶ－１呈阳性，而健侧１０份中３份阳性；角膜植片１０只有７只阳性，术侧三叉神经节未检测到ＨＳＶ－１ＤＮＡ。由此提示，单纯疱疹病毒Ｉ型（ＨＳＶ－１）既可在同侧三叉神经节形成潜伏感染，又可能在对侧三叉神经节形成潜伏，而且角膜极可能为ＨＳＶ－１的另一潜伏部位
Pure strains of New Zealand white rabbits were inoculated HSV-lstokek strain, the successful establishment of herpes simplex virus keratitis model. 45 days after self-healing, the cornea of the original lesion as a donor, healthy New Zealand white rabbits as recipients, percutanous keratoplasty (PKP) at the same time, take the affected side of 10 and 10 healthy trigeminal ganglion, the application of polymerase chain HSV-1 DNA was detected by Polymerase Chain Reaction (PCR). Fifty days after operation, 10 corneal allografts and 10 trigeminal ganglions were used for the same test. The results showed that 8 HSV-1 positive in 10 affected trigeminal ganglions and 3 positive ones in 10 healthy corneas were positive; 10 of 7 corneal grafts were positive and no HSV-1 DNA was detected in the trigeminal ganglion. Thus, HSV-1 can induce latent infection in the ipsilateral trigeminal ganglion and latent in the contralateral trigeminal ganglion, and the cornea is most likely another latent site of HSV-1