论文部分内容阅读
目的观察创面负压治疗对糖尿病足患者肉芽组织bFGF的影响。方法将2015年2月—2017年2月期间50例在该院确诊的糖尿病足患者随机分为常规组(常规治疗)与干预组(创面负压治疗),治疗1周,比较两组bFGF表达的情况及皮损愈合面积。结果 (1)HE染色:干预组治疗后HE染色能见新鲜肉芽组织,常规组相对较少;组织Masson染色:干预组能见大量胶原纤维沉积,常规组治疗前后变化不明显;免疫组化法:干预组治疗后bFGF表达水平与治疗前对比升高明显,常规组则无明显变化;Western blotting法:治疗后干预组肉芽组织bFGF表达水平与治疗前相比升高明显,常规组治疗前后变化不明显(P>0.05);(2)干预组治疗5、10 d与15 d,创面愈合面积均大于常规组,差异有统计意义(P<0.05)。结论创面负压治疗可上调糖尿病足患者bFGF表达,促进创面愈合。
Objective To observe the effect of negative pressure wound therapy on granulation tissue bFGF in patients with diabetic foot. Methods Fifty patients with diabetic foot diagnosed in this hospital from February 2015 to February 2017 were randomly divided into routine treatment group and intervention group (negative pressure wound treatment) for 1 week. The bFGF expression was compared between the two groups The situation and the lesion healing area. Results: (1) HE staining: fresh granulation tissue could be seen by HE staining after treatment in the intervention group, there was relatively little in the conventional group; Masson staining: a large amount of collagen fibers could be seen in the intervention group, but not in the conventional group; : The expression of bFGF in the intervention group increased significantly compared with that before treatment, but there was no significant change in the routine group. The expression of bFGF in granulation tissue of the intervention group increased significantly after treatment compared with that before treatment (P> 0.05). (2) The wound healing area in the intervention group was significantly higher than that in the conventional group at 5, 10, and 15 days after treatment (P <0.05). Conclusion The negative pressure wound treatment can up-regulate bFGF expression in diabetic foot patients and promote wound healing.