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细菌外膜蛋白与细菌对异丙醇耐受关系密切,但迄今为止尚未见相关研究.本文首先采用基于双向电泳(two dimensional electrophoresis,2-DE)的蛋白质组学技术,研究E.coli K-12 BW25113在有无异丙醇条件下外膜蛋白表达的差异.结果发现,外膜蛋白LamB、FadL和OmpC以及OmpT、Tsx、OmpA和OmpF在异丙醇应激条件下表达量分别上调和下调.然后通过基因敲除、补救和高表达等功能基因组学的方法,探讨这些功能外膜蛋白在异丙醇应激耐受中所起的作用,发现LamB、OmpA和OmpC在E.coli K-12 BW25113对异丙醇耐受过程中起到更重要的作用.最后,对EnvZ/OmpR双组分信号转导系统在对异丙醇耐受中的作用进行了研究,证实EnvZ/OmpR双组分信号转导系统确实参与细菌对异丙醇的耐受.因此,外膜蛋白的改变和EnvZ/OmpR双组分信号转导系统的调节是革兰氏阴性细菌对异丙醇耐受的一种重要机制。
Bacterial outer membrane proteins and bacteria are closely related to isopropanol tolerance, but so far no relevant research has been reported.In this paper, proteomic techniques based on two dimensional electrophoresis (2-DE) 12 BW25113 in the presence or absence of isopropanol.The results showed that the expressions of outer membrane proteins LamB, FadL and OmpC and OmpT, Tsx, OmpA and OmpF were upregulated and downregulated under isopropanol stress Then functional genomics methods such as gene knockout, remedy and high expression were used to explore the role of these functional outer membrane proteins in isoproterenol stress tolerance and found that the functions of LamB, OmpA and OmpC in E.coli K- 12 BW25113 plays a more important role in isopropanol tolerance.Finally, the effect of EnvZ / OmpR two-component signal transduction system on isopropanol tolerance was studied and it was demonstrated that the EnvZ / OmpR double cohort The sub-signaling system does participate in bacterial tolerance to isopropanol Therefore, alterations in the outer membrane protein and regulation of the EnvZ / OmpR two-component signaling system are one of the gram-negative bacteria resistant to isopropanol Important mechanism.