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以大猿叶虫Colaphellus bowringi Baly4个地理种群的基因组DNA为材料,进行RAPD分析。从80条引物中筛选出11条稳定性好、多态性高的引物进行扩增,共得到65个扩增位点,53个多态位点,Nei氏遗传多样性指数为0.1049～0.2061,Shannon多样性指数为0.1641～0.3167。结果表明所分析的大猿叶虫遗传变异很高,其中江西龙南种群遗传变异最小,山东泰安种群遗传变异最高。种群间的遗传距离范围为0.0636～0.3200,其中江西龙南种群和江西修水种群间的遗传距离最小,哈尔滨种群与江西龙南种群间的遗传距离最大,种群遗传距离的大小与其相对地理距离的远近吻合。结果提示种群遗传距离的大小与它们生物学上的相似性有关联。
The genomic DNAs of four geographical populations of Colaphellus bowringi Baly were used as RAPD markers. Eleven primers with good stability and high polymorphism were screened from 80 primers for amplification. A total of 65 amplification loci and 53 polymorphic loci were obtained. The Nei’s genetic diversity index was 0.1049-0.2061, Shannon’s diversity index was 0.1641 ~ 0.3167. The results showed that the genetic variation of the large-scale leaf-worm was very high, among which the genetic variation was the smallest in Longnan, Jiangxi and the highest in Tai’an, Shandong. The genetic distance ranged from 0.0636 to 0.3200. Among them, the genetic distance was the smallest among Longnan population in Jiangxi province and Xiushui population in Jiangxi province. The genetic distance between Harbin population and Longnan population in Jiangxi province was the largest. The population genetic distance was consistent with its relative geographic distance . The results suggest that the genetic distance between populations is related to their biological similarity.