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选用我省济宁产的并经实验驯化的中华按蚊为实验材料。将中华按蚊生活史中卵、幼虫,蛹及成蚊放置于恒温的条件下,成蚊饲养环境的相对湿度为80～90％。恒温分级选择16、19、22、25、28、31±0.5等6种摄氏度。 卵、幼虫和蛹的观察:选用同批卵各200粒为一组,盛放在水容器中,分别放在6个温度下观察其孵化情况。选用同批孵出的一龄幼虫各100个,同样在不同温度下饲养,饲养方法常规进行,逐日记录
Selection of our province Jining produced and experimentally acclimated Chinese Anopheles as the experimental material. The life history of Anopheles sinensis eggs, larvae, pupa and adult mosquitoes placed in a constant temperature conditions, adult mosquitoes feeding environment relative humidity of 80 ~ 90%. Temperature grading selection 16,19,22,25,28,31 ± 0.5 and other six kinds of degrees Celsius. Eggs, larvae and pupa observation: The same batch of eggs each 200 as a group, placed in water containers, respectively, on the 6 observed the incubation temperature. Selected the same batch hatched first instar larva of 100, the same temperature feeding, feeding methods routinely, daily record