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目的探讨白藜芦醇对氧化型低密度脂蛋白(oxLDL)所致PC12细胞损伤的保护作用。方法白藜芦醇预处理细胞,再加入不同浓度oxLDL,通过噻唑蓝(MTT)实验、乳酸脱氢酶(LDH)释放实验、TUNEL实验及半胱氨酰天冬氨酸特异性蛋白酶3(Caspase3)活性测定,观察白藜芦醇对细胞存活率、细胞膜通透性、细胞核及Caspase3活性的影响。结果10mg/LoxLDL组细胞存活率、LDH释放率、TUNEL阳性细胞数、Caspase3活性分别为(62±3)%、(23±3)%、(26±5)%和(0811±0049)mol·min-1·μg-1;10mg/LoxLDL+50μmol/L白藜芦醇组分别为(84±7)%、(13±4)%、(12±4)%和(0553±0048)mol·min-1·μg-1,两组比较差异有统计学意义。结论白藜芦醇具有减轻oxLDL致PC12细胞的毒性作用;对PC12细胞具有神经保护作用。
Objective To investigate the protective effect of resveratrol on the injury of PC12 cells induced by oxidized low-density lipoprotein (oxLDL). Methods Resveratrol was used to pretreat cells, and then different concentrations of oxLDL were added to pass MTT assay, lactate dehydrogenase (LDH) release assay, TUNEL assay and cysteinyl aspartate-specific protease 3 (Caspase 3). ) Activity assay to observe the effects of resveratrol on cell viability, cell membrane permeability, nucleus and Caspase3 activity. Results The cell survival rate, LDH release rate, TUNEL positive cell number, and Caspase 3 activity in 10 mg/LoxLDL group were (62±3)%, (23±3)%, (26±5)%, and (0811±0049) mol. The concentration of min-1·μg-1; 10mg/LoxLDL+50μmol/L resveratrol was (84±7)%, (13±4)%, (12±4)% and (0553±0048) mol·respectively. Min-1·μg-1, the difference between the two groups was statistically significant. Conclusion Resveratrol can reduce the toxicity of PC12 cells induced by oxLDL and has neuroprotective effect on PC12 cells.