双波长等吸收点法测定复方甲硝唑口腔药膜中甲硝唑的含量

• 作 者: 张立华 初晓 王本坚 王少华
• 机 构: 青岛市立医院,青岛市立医院,青岛市立医院,青岛市立医院 青岛 266011,青岛 266011,青岛 266011,青岛 266011
• 出 处: 中国海洋药物
• 发表日期: 1997年02期
• 关键词: 口腔药膜 复方甲硝唑 达克罗宁 等吸收点 药物检测 波长范围 处方量 甲哨唑 吸收度 照分光光度法

报道了复方甲哨唑口腔药膜中甲硝唑的含量测定。该口腔药膜作为复方制剂含有多种药物,除了甲硝唑、达克罗宁外,其它药物在240～320nm几乎没有紫外吸收。因为达克罗宁在288nm、274.5nm两波长处具有等吸收,且甲硝唑吸收度差值△A足够大,因此应用双波长等吸收点法于此两波长处测定甲硝唑的含量。以消除达克罗宁对主要成分甲硝唑测定的影响。实验结果表明,双波长等吸收点法简单、快捷、重现性好,适合药物检测应用。

The content of metronidazole in compound metostasol oral film was reported. The oral film as a compound containing a variety of drugs, in addition to metronidazole, dacronin, other drugs in the almost no UV absorption in the 240 ~ 320nm. Since dacronin has equal absorption at 288 nm and 274.5 nm, and the difference in metronidazole absorption, ΔA, is large enough, two-wavelength isoabsorption method is used to determine metronidazole at these two wavelengths. To eliminate the impact of dacronin on the determination of the main ingredient metronidazole. Experimental results show that the dual wavelength absorption point method is simple, fast, reproducible, suitable for drug testing applications.