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目的:用P-gp的底物~(99m)Tc-MIBI评价中药钙通道阻滞剂川芎嗪(TMP)对乳腺癌MCF-7/ADR细胞耐多药的逆转作用。方法:以经典逆转剂维拉帕米和MCF-7/WT细胞作对照,各组均加入~(99m)Tc-MIBI,分别测定逆转剂作用前后细胞内(C_(in))和上清液(C_(out))中的放射性活度的比值。结果:在加入逆转剂之前MCF-7/WT和MCF-7/ADR细胞株间~(99m)Tc-MIBI聚集的差别为33倍,加入逆转剂 60min后二者间的差别为3.8倍。结论:~(99m)Tc-MIBI的变化可以反映耐多药的逆转,TMP可部分逆转MCF-7/ADR的耐多药性。
OBJECTIVE: To evaluate the reversal of multidrug-resistant multidrug resistance in MCF-7 / ADR breast cancer cells by ligating tetramethylpyrazine (TMP), a calcium channel blocker of P-gp, to 99m Tc-MIBI. Methods: Verapamil and MCF-7 / WT cells were used as control. The levels of ~ (99m) Tc-MIBI were measured in each group before and after treatment with reversal agents. The levels of C_ (in) and supernatant (C_ (out)) of radioactivity in the ratio. RESULTS: The difference in aggregation of ~ (99m) Tc-MIBI between MCF-7 / WT and MCF-7 / ADR cell lines was 33-fold before addition of a reversal agent and 3.8-fold after addition of a reversal agent for 60 min. CONCLUSION: The change of ~ (99m) Tc-MIBI can reflect the reversal of MDR and TMP can partially reverse the multidrug resistance of MCF-7 / ADR.