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目的 :了解杭州市淋病奈瑟菌质粒携带及质粒谱型分布情况。方法 :采用碱裂解法对门诊 2 0 0 0年 1月～ 2 0 0 1年 10月分离的 2 0 7株淋病奈瑟菌进行了质粒抽提及质粒谱分型研究 ,并对菌株的青霉素耐药现象和耐药性质粒的关系进行探讨。结果 :2 0 7株淋病奈瑟菌中 194株 (93 .7% )检见质粒带 ,其中含一条质粒带的 112株 (5 4.11% ) ,含两条质粒带的 12株 (5 .8% ) ,含三条带的 70株 (3 3 .82 % ) ,尚有 13株(6.2 8% )未检测到质粒。以 E.coli V5 17细菌质粒作分子量标准 ,测得这些分子量分别为 2 .6、4.5、和 2 4.5Md。质粒谱型以 2 .6+ 4.5 + 2 4.5 Md(3 3 .82 % )多见。结论 :杭州地区质粒酶切图谱的分析研究有助于淋病的治疗和防治 ,这将对该地区淋病奈瑟菌的分子流行病学调查和淋病监控提供依据
Objective: To understand the distribution of plasmid carriers and plasmid profiles of Neisseria gonorrhoeae in Hangzhou. Methods: Neisseria gonorrhoeae strains were isolated from outpatients from January 2000 to October 2001 by using alkaline lysis method. Plasmid extraction and plasmid typing were carried out on the strains of penicillin Drug resistance and drug resistance of the relationship between the plasmid to be explored. Results: Of the 207 strains of Neisseria gonorrhoeae, 194 strains (93.7%) were found plasmid, including 112 strains (5 4.11%) of one plasmid and 12 strains (5.8 %), 70 strains (33.82%) containing three bands and 13 strains (6.2%) did not detect plasmids. The E. coli V5 17 bacterial plasmids were used as molecular weight standards and these molecular weights were found to be 2.6, 4.5, and 4.5 Md, respectively. Plasmid patterns were more common at 2.6 + 4.5 + 2 4.5 Md (33.82%). Conclusion: The analysis of the plasmid restriction map in Hangzhou area is helpful for the treatment and prevention of gonorrhea, which will provide a basis for the molecular epidemiological investigation and gonorrhea surveillance of Neisseria gonorrhoeae in the area