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单克隆抗体NCC-ST-439是用人胃癌细胞株St-4作免疫原制成的单克隆抗体,在多种癌组织中发现率较高。为了探讨其对癌症的血清诊断意义,作者开发了夹层放射免疫测定法(SRIA),现报告如下。对象和方法对象为健康供血者233例,正常妊娠妇女119例,各种恶性肿瘤284例和良性疾病119例。鼠腹腔内投予经降植烷(Pristane)处理的杂交细胞瘤后,将所得腹水用FPLC高速液相层析系统制备出抗体。用乳过氧化物酶/葡萄糖氧化酶法制备~(125)I标记二次抗体,其抗体活性比为3～4mCi/mg蛋白。对照组的非标志抗体是以磷酸盐水代替~(125)I。用直径为
The monoclonal antibody NCC-ST-439 is a monoclonal antibody made by using human gastric cancer cell line St-4 as an immunogen and has a high discovery rate in various cancer tissues. In order to investigate its significance for the diagnosis of cancer, the authors developed a sandwich radioimmunoassay (SRIA), which is reported below. The subjects and methods were 233 healthy blood donors, 119 normal pregnant women, 284 malignant tumors and 119 benign diseases. After intraperitoneal administration of pristane-treated hybridomas to mice, the ascitic fluids were prepared using FPLC high-speed liquid chromatography system. The 125I-labeled secondary antibody was prepared by the lactoperoxidase/glucose oxidase method and its antibody activity ratio was 3 to 4 mCi/mg protein. The non-marker antibody in the control group was phosphate water instead of ~(125)I. With a diameter of