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目的 :探讨不同亚型的腹腔巨噬细胞对调节性T细胞(regulatory T cell,Treg)体外诱导过程的影响及可能机制。方法:按Treg的不同诱导条件,分为不加巨噬细胞的正常Treg诱导组(no M组)、原代巨噬细胞影响组(M0组)、M1型巨噬细胞影响组(M1组)、M2型巨噬细胞影响组(M2组)。采用流式细胞术检测不同诱导条件下Foxp3表达以及效应性T细胞增殖情况,HE染色检测不同条件诱导的Treg处理肝脏缺血再灌注模型小鼠的肝脏病理,全自动生化分析仪分析各组小鼠血清ALT水平。结果:与no M组相比,M0组与M2组诱导Foxp3表达明显降低(P<0.01),而M1组对Fxop3诱导无明显影响。此外,M0与M2组Treg体外抑制功能以及体内保护功能明显低于no M和M1组(P<0.01),而这种作用的实现依赖细胞之间的接触性作用(P<0.05)。结论 :腹腔巨噬细胞能够影响Treg的体外诱导,不同亚型腹腔巨噬细胞对Treg影响不同,共同为机体免疫平衡发挥着关键性作用。
Objective: To investigate the effects of different subtypes of peritoneal macrophages on the induction of regulatory T cell (Treg) in vitro and its possible mechanism. Methods: According to the different inducing conditions of Treg, we divided into normal Treg induction group without macrophages (no M group), primary macrophage influence group (M0 group), M1 macrophage influence group (M1 group) , M2 macrophages (M2 group). The expression of Foxp3 and the proliferation of effector T cells under different induction conditions were detected by flow cytometry. The liver pathology of liver ischemic-reperfusion model mice treated with Treg induced by different conditions were detected by HE staining. The results of automatic biochemical analyzer Rat serum ALT levels. Results: Compared with no M group, Foxp3 expression was significantly decreased in M0 group and M2 group (P <0.01), while M1 group had no significant effect on Fxop3 induction. In addition, the inhibitory function and in vivo protective function of Treg in M0 and M2 groups were significantly lower than those in no M group and M1 group (P <0.01). However, this effect was dependent on the contact between cells (P <0.05). CONCLUSION: Peritoneal macrophages can affect the induction of Tregs in vitro. Different subtypes of peritoneal macrophages have different effects on Tregs and play a key role in immune balance.