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【目的】研究黄瓜花叶病毒病(CMV)侵染胁迫下黄瓜重要功能基因表达和代谢途径响应。【方法】在结合气体交换和叶绿素荧光参数及抗性相关酶活性测定的基础上,应用农业部园艺植物生长发育与品质调控重点开放实验室自主开发的黄瓜cDNA芯片研究黄瓜在CMV侵染胁迫下的基因表达谱变化,并对其中5个具有代表性的基因通过实时荧光定量PCR(QRT—PCR)进行检测,以验证cDNA芯片杂交结果的可靠性。【结果】共获得67个差异表达显著的基因,其中42个基因下调表达,25个基因上调表达。这些差异表达的基因涉及了许多重要代谢途径。许多与光合作用、氮同化相关的基因受到明显的抑制;而一些防御相关基因和信号传导相关基因则诱导表达;同时,也发现了一些与CMV胁迫相关的功能未知基因或未有任何功能信息的基因。【结论】CMV胁迫引发了黄瓜代谢发生一系列复杂的适应性变化,PR1-1a等基因可能在黄瓜防御CMV侵染过程发挥着重要作用。
【Objective】 The objective of this study was to investigate the expression of important functional genes and their metabolic pathways under cucumber mosaic virus (CMV) infection. 【Method】 Based on the combination of gas exchange and chlorophyll fluorescence parameters and enzyme activity determination, the cucumber cDNA chip developed by the Key Laboratory of Horticultural Plant Growth and Quality Control of Ministry of Agriculture was used to study the effects of CMV infection on , And 5 representative genes were detected by real-time fluorescence quantitative PCR (QRT-PCR) to verify the reliability of cDNA microarray hybridization results. 【Result】 A total of 67 differentially expressed genes were obtained, of which 42 genes were down-regulated and 25 genes were up-regulated. These differentially expressed genes involved many important metabolic pathways. Many genes involved in photosynthesis and nitrogen assimilation were significantly inhibited, while some defense-related genes and signal transduction-related genes induced expression. At the same time, some genes with unknown function related to CMV stress were also found or there was no functional information gene. 【Conclusion】 CMV stress induces a series of complex adaptive changes in cucumber metabolism. The genes such as PR1-1a may play an important role in cucumber defense against CMV infection.