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从来自春大麦品种“如车”成熟胚的愈伤组织中,挑选出适于悬浮培养的松脆型胚性愈伤组织,在短期内建立胚性细胞悬浮系。此系酶解后分离出的原生质体在修改的MS培养基上能够持续分裂形成愈伤组织。将其直接转至分化培养基上获得结构紧密的胚性愈伤组织并再生白化苗.
From callus from mature embryos of spring barley varieties such as “car”, the embryogenic callus of embryogenic cells was selected in a short time for the selection of embryogenic callus, which is suitable for suspension culture. Protoplasts isolated after enzymolysis of this strain can continue to divide to form callus on the modified MS medium. Directly transferred to differentiation medium to obtain close structure of embryogenic callus and regenerate albino seedlings.