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【目的】通过检测印迹基因IGF2在先兆子痫产妇胎盘中的特殊等位基因的表达,探讨IGF2印迹状态与先兆子痫发病的关系。【方法】收集经剖宫产的正常足月妊娠及先兆子痫产妇胎盘,从胎盘中提取基因组DNA,经PCR扩增、Apa1酶切筛选杂合子。提取杂合子胎盘中的总RNA并逆转录合成cDNA,cDNA经PCR扩增、内切酶消化,以评价IGF2特殊等位基因的表达。【结果】IGF2在正常足月胎盘中呈印迹状态;在先兆子痫胎盘中,IGF2为单等位基因表达,无杂合子状态。【结论】在先兆子痫胎盘中未发生IGF2的印迹丢失。
【Objective】 To investigate the relationship between the status of IGF2 imprinting and the incidence of preeclampsia by detecting the expression of special alleles of imprinted gene IGF2 in maternal placenta of preeclampsia. 【Methods】 The normal fetus pregnancy and preeclampsia maternal placenta collected through cesarean section were collected. The genomic DNA was extracted from the placenta. After PCR amplification and Apa1 digestion, the heterozygotes were screened. The total RNA in the hybrid placenta was extracted and cDNA was reverse transcribed. The cDNA was amplified by PCR and digested with endonuclease to evaluate the expression of special alleles of IGF2. 【Results】 IGF2 was imprinted in normal term placenta. In preeclamptic placenta, IGF2 was expressed as a single allele with no heterozygotes. [Conclusion] There is no loss of imprinting of IGF2 in pre-eclamptic placenta.