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在热带流行区,用冻干虫卵作环卵沉淀试验,由于缺乏复杂的实验设备而受到限制。作者试图应用福尔马林固定的虫卵来进行环卵沉淀试验。日本血吸虫卵(菲律宾株),取自感染血吸虫后4～7周的ddY小鼠肝脏,按Kamiya等(1980)消化方法收集虫卵。纯化的虫卵大部分经5%福尔马林溶液(内含1.85%甲醛)固定后,于室温下保存约2年。另一部分虫卵冻干保存于4℃冰箱相同时间作为对照。福尔马林固定的虫卵在使用前2天内用pH7.2的PBS溶液充分洗涤数次。抗血清为3份混合的感染兔血清(家兔接种600条
In tropical endemic areas, freeze-dried eggs are used as eggs for egg-precipitation tests, which are limited by the lack of sophisticated laboratory equipment. The authors attempted to use the formalin-fixed eggs to carry out the ESR test. Schistosoma japonicum eggs (Philippines strain), collected from the liver of ddY mice 4 to 7 weeks after infection with schistosomiasis, were collected by the method of Kamiya et al. (1980) digestion. Purified eggs were mostly preserved in 5% formalin solution (containing 1.85% formaldehyde) and stored at room temperature for about 2 years. Another part of the eggs were freeze-dried in 4 ℃ refrigerator at the same time as a control. Formalin-fixed eggs were washed several times with PBS solution, pH 7.2, two days prior to use. The antiserum was 3 mixed inactivated rabbit sera (600 rabbits inoculated