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【目的】探讨蛋白酪氨酸磷酸酶1B（PTP1B）和瘦素、脂联素在继发性闭经患者外周血清中的表达水平及意义。【方法】选取60例在哈尔滨医科大学附属第二医院诊断为继发性闭经的患者为观察组，其中30例闭经合并肥胖症，30例闭经无肥胖症；另选取60例体检月经正常者为对照组，其中30例合并肥胖症，30例无肥胖症。采用酶联免疫吸附实验（ELISA ）检测两组患者血清中 PT P1B、瘦素和脂联素的水平。【结果】闭经合并肥胖症组患者PTP1B浓度（86．24±17．58） ng/L与瘦素浓度（16．14±2．67） ng/L明显高于对照组中合并肥胖症组的患者（54．66±13．27） ng/L ，（10．10±1．93） ng/L（均 P＜0．05）；而脂联素浓度（4．71±2．64） mg/L明显低于对照组中合并肥胖症组的患者（10．26±3．13） mg/L（ P＜0．05）。闭经合并肥胖症组中PTP1B浓度与闭经无肥胖症组间差异并不明显（73．97±10．25） ng/L （ P＞0．05）；而瘦素浓度明显升高（6．53±3．25） ng/L ，脂联素浓度明显降低（11．76±3．58）mg/L（均 P＜0．05），与肥胖症有关。闭经合并肥胖症组患者中PTP1B与体质量指数（BM I ）、瘦素的表达水平呈正相关，与脂联素的表达水平呈负相关。【结论】继发性闭经合并肥胖症患者与血清中PT P1B、瘦素和脂联素的表达水平在促进疾病的发生发展中有重要意义。“,”[Objective]To explore the serum levels of protein tyrosine phosphatase 1B(PTP1B) ,leptin and adiponectin in patients with secondary amenorrhea .[Methods]A total of 60 patients diagnosed as secondary a-menorrhea in second affiliated hospital of Harbin medical university including 30 patients with obesity and 30 patients without obesity were selected as case group .Other 60 patients with normal menstrual including 30 pa-tients with obesity and 30 patients without obesity were selected as control group .Serum levels of PTP1B , leptin and adiponectin were measured by enzyme linked immunosorbent assay (ELISA) .[Results]Serum levels of PTP1B and leptin in amenorrhea with obesity group were (86 .24 ± 17 .58)ng/L and (16 .14 ± 2 .67)ng/L re-spectively ,which were obviously higher than those in patients with obesity in control group [(54 .66 ± 13 .27) ng/L and (10 .10 ± 1 .93)ng/L ,respectively)](all P <0 .05) ,while serum level of adiponectin was obviously lower than that of patients with obesity in control group [(4 .71 ± 2 .64)mg/L vs .(10 .26 ± 3 .13)mg/L]( P0 .05) ,while serum level of leptin was ob-viously increased[(6 .53 ± 3 .25) ng/L] and serum level of adiponectin was obviously decreased[(11 .76 ± 3 .58) mg/L](all P<0 .05) .Serum levels of leptin and adiponectin were related with obesity .PTP1B in amenorrhea with obesity patients was positively correlated with body mass index (BMI) and leptin ,but negatively correla-ted with adiponectin .[Conclusion] Serum levels of PTP1B ,leptin and adiponectin in obesity patients with a-menorrhea are of vital significance in promoting the occurrence and development of the disease .