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目的探讨ω-3鱼油脂肪乳对乌头碱诱发大鼠心律失常的影响及相关机制。方法 50只雄性大鼠随机分为ω-3鱼油脂肪乳组和对照组,每组25只。实验前15 d,ω-3鱼油脂肪乳组大鼠隔日经尾静脉注射ω-3鱼油脂肪乳2 mL.kg-1;对照组注射等量的生理盐水。应用乌头碱诱发心律失常大鼠模型,观察记录2组大鼠室性期前收缩、室性心动过速出现的时间及心律失常评分,Western blot检测大鼠心肌组织缝隙连接蛋白43(CX43)的表达。结果ω-3鱼油脂肪乳组大鼠室性期前收缩和室性心动过速出现时间均显著长于对照组(P<0.05)。静脉注射乌头碱后1 min,2组大鼠心律失常评分比较差异无统计学意义(P>0.05);静脉注射乌头碱后3、5、7 min,ω-3鱼油脂肪乳组大鼠心律失常评分均低于对照组(P<0.05)。ω-3鱼油脂肪乳组大鼠心肌组织中CX43蛋白的表达高于对照组(P<0.05),但2组大鼠心肌组织中去磷酸化CX43蛋白表达比较差异无统计学意义(P>0.05)。结论ω-3鱼油脂肪乳可能通过促进CX43的磷酸化改善乌头碱诱导的大鼠心律失常。
Objective To investigate the effects of omega-3 fish oil emulsion on aconitine-induced arrhythmia in rats and its underlying mechanisms. Methods Fifty male rats were randomly divided into ω-3 fish oil-fat emulsion group and control group, with 25 rats in each group. Fifteen days before the experiment, the rats in the omega-3 fish oil-fat emulsion group were injected 2 mL.kg-1 omega-3 fish oil emulsion through the tail vein every other day. The control group received the same amount of saline. Application of aconitine induced arrhythmia rat model was observed and recorded two groups of rats ventricular contraction, ventricular tachycardia appeared time and arrhythmia score, Western blot detection of myocardial tissue connexin 43 (CX43) expression. Results The onset time of ventricular premature ventricular contraction and ventricular tachycardia in omega-3 fish oil emulsion was significantly longer than that in control group (P <0.05). After intravenous injection of aconitine for 1 min, there was no significant difference in arrhythmia score between the two groups (P> 0.05); 3,5,7 min after intravenous injection of aconitine, Arrhythmia scores were lower than the control group (P <0.05). The expression of CX43 protein in myocardium of omega-3 fish oil fat emulsion group was higher than that of the control group (P <0.05), but there was no significant difference in the expression of dephosphorylated CX43 protein between the two groups (P> 0.05 ). Conclusion Omega-3 fish oil emulsion may improve aconitine-induced arrhythmia in rats by promoting the phosphorylation of CX43.