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溶解性有机物的荧光组份由于受到金属离子的影响其荧光强度受到变化,从而为溶解性有机物的定量分析带来挑战。利用三维荧光结合平行因子分析方法研究了Cu(Ⅱ),Fe(Ⅲ),Ni(Ⅱ),Sr(Ⅱ),Hg(Ⅱ),K(Ⅰ),Mg(Ⅱ)和Mn(Ⅱ)八个金属离子对典型溶解性有机物荧光组分的荧光猝灭作用,五个水样来源各不相同。实验表明水样被平行因子成功分解为三个荧光组分(色氨酸、腐殖酸、富里酸),这三个荧光组份的荧光得分随着Fe(Ⅲ),Cu(Ⅱ),Hg(Ⅱ)和Ni(Ⅱ)浓度的增加呈线性或指数下降。在这四种离子中,Fe(Ⅲ)和Cu(Ⅱ)对腐殖酸和富里酸的荧光猝灭作用明显大于Hg(Ⅱ)和Ni(Ⅱ)。Sr(Ⅱ),K(Ⅰ),Mg(Ⅱ)和Mn(Ⅱ)基本没有影响。其中Fe(Ⅲ)对于三个荧光组分的荧光猝灭都有较好的分析。由于只考虑Fe(Ⅲ)和Cu(Ⅱ)的影响,对于含有金属离子的饮用水的荧光强度的校正曲线也被建立。不同来源的饮用水中荧光组份的荧光得分的衰减规律也是不一样的,因此水样的来源也是测量时需要考虑的因素。实验证实了金属离子对溶解性有机物的荧光猝灭作用以及其他因素比如Fe(Ⅲ)和Cu(Ⅱ)和溶解性有机物本身的多样性都是我们荧光测量典型荧光组份时需要考虑的。表明三维荧光结合平行因子分析方法是一个有效的准确测量溶解性有机物荧光组份的工具。
Fluorescent components of dissolved organic matter are subject to changes in fluorescence intensity due to the influence of metal ions, thereby presenting a challenge for the quantitative analysis of dissolved organic substances. The effects of Cu (Ⅱ), Fe (Ⅲ), Ni (Ⅱ), Sr (Ⅱ), Hg (Ⅱ), K (Ⅰ), Mg (Ⅱ) and Mn (Ⅱ) A metal ions on the fluorescence of typical dissolved organic compounds fluorescence quenching, five water samples vary from source. Experiments show that the water sample is successfully decomposed into three fluorescent components (tryptophan, humic acid, fulvic acid) by the parallel factor. Fluorescence scores of the three fluorescent components with the Fe (Ⅲ), Cu (Ⅱ), Hg (Ⅱ) and Ni (Ⅱ) concentrations increased linearly or exponentially. Among these four ions, the fluorescence quenching effects of Fe (Ⅲ) and Cu (Ⅱ) on humic acid and fulvic acid were significantly greater than those of Hg (Ⅱ) and Ni (Ⅱ). There was almost no effect of Sr (Ⅱ), K (Ⅰ), Mg (Ⅱ) and Mn (Ⅱ). Among them, Fe (Ⅲ) has a good analysis of the fluorescence quenching of the three fluorescent components. Since only the effects of Fe (III) and Cu (II) are considered, a calibration curve for the fluorescence intensity of drinking water containing metal ions is also established. Fluorescent components of drinking water from different sources of decay of fluorescence score is not the same, so the source of water samples is also measured when the factors to be considered. Experiments confirmed that the metal ions on the fluorescence quenching of dissolved organic matter and other factors such as Fe (Ⅲ) and Cu (Ⅱ) and the diversity of dissolved organic matter itself are typical of our fluorescent fluorescence component to be considered. Indicating that the three-dimensional fluorescence combined with parallel factor analysis method is an effective and accurate tool for measuring the fluorescence of dissolved organic compounds.