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目的：研究重组ＦＮ多肽ＣＨ５０在体内激活巨噬细胞及其抗肿瘤作用的特点。方法：体内注射ＣＨ５０和／或转染ＩＦＮ－γ基因，检测巨噬细胞产生的各种因子，测定荷瘤鼠体内肿瘤生长速度。结果：ＣＨ５０单独作用可促进巨噬细胞产生ＮＯ，ＴＮＦ，ＩＬ－１等因子，但激活作用较慢。注射ＣＨ５０和转染ＩＦＮ－γ基因不分先后，均可在体内协同作用，迅速激活巨噬细胞。单独注射ＣＨ５０能够抑制肿瘤结节的形成，其抑制作用呈剂量依赖关系；低剂量ＣＨ６０对＜１ｍｍ的肿瘤结节已有很强的抑制作用，较大剂量时对＞１ｍｍ的肿瘤结节也有很强的抑制作用。ＣＨ５０与ＩＦＮ－了在体内的协同作用使其抑制体内肿瘤生长的作用更强。结论：ＣＨ５０与ＩＦＮ－γ作为体内巨噬细胞激活的双信号因子在肿瘤治疗中具有极大的应用潜力。
Objective: To study the characteristics of the recombinant FN polypeptide CH50 activating macrophages in vivo and its anti-tumor effect. METHODS: Intramuscular injection of CH50 and/or transfection of IFN-γ gene were performed to detect various factors produced by macrophages. The tumor growth rate in tumor-bearing mice was measured. Results: CH50 alone could promote the production of NO, TNF, IL-1 and other factors in macrophages, but the activation was slower. Injecting CH50 and transfecting IFN-γ gene in no particular order can all act synergistically in vivo to rapidly activate macrophages. Injection of CH50 alone can inhibit the formation of tumor nodules with a dose-dependent inhibitory effect; low-dose CH60 has a strong inhibitory effect on tumor nodules <1 mm, and tumors with >1 mm are also very effective at higher doses. Strong inhibitory effect. The synergistic effect of CH50 and IFN-γ in vivo leads to stronger inhibition of tumor growth in vivo. Conclusion: CH50 and IFN-γ have great potential as dual signalling factors for activation of macrophages in vivo.