论文部分内容阅读
转基因抗草甘膦棉花喷施草甘膦可引起花药败育,但关于草甘膦诱导抗草甘膦棉花花药败育过程中的生化变化尚未见报道。本文以转基因抗草甘膦棉花新品系N95为试验材料,用0.2%草甘膦喷洒棉株为处理,以不喷为对照。试验结果表明:(1)草甘膦处理的植株其花粉活力很低,平均只有3%;而未经处理的植株花粉活力则平均高达86%,两者差异达极显著水平,显示抗草甘膦棉花喷施草甘膦后,可导致其花粉雄性不育。(2)分期取样,测定花粉4个发育时期内源激素含量,结果显示处理株花药中生长素含量始终低于对照,平均只有对照株中的43.8%~64.3%;脱落酸的含量始终高于对照,平均为对照株含量的1.33~1.87倍;玉米素的含量始终低于对照,平均只有对照株的39.6%~57.1%;赤霉素含量也始终低于对照,平均只有对照株的50.5%~64.9%。(3)处理株与对照株花药中氨基酸含量的检测结果发现:苯丙氨酸极显著低于对照,赖氨酸、组氨酸、缬氨酸和蛋氨酸的含量显著低于对照,谷氨酸含量则显著高于对照。根据以上研究结果推断:花药中生长素的降低、脱落酸的增加、细胞分裂素及赤霉素含量的不足是导致花药败育的基本生化因子。另外,苯丙氨酸含量极显著偏低,赖氨酸、组氨酸、缬氨酸的含量显著偏低以及谷氨酸含量显著偏高,也与棉花花药的败育存在不同程度的关联。
Transgenic glyphosate-resistant cotton plants can cause anther abortion when they are sprayed with glyphosate, but no reports have been reported on the biochemical changes induced by glyphosate-induced abortion of glyphosate-resistant cotton anthers. In this paper, a new line of transgenic glyphosate-resistant cotton N95 was used as experimental materials, treated with 0.2% glyphosate sprayed cotton plants without spraying as a control. The results showed that: (1) The pollen viability of glyphosate-treated plants was very low, with an average of only 3%; while that of untreated plants was as high as 86%, with significant differences between the two Phosphine cotton after spraying glyphosate can cause male sterility of its pollen. (2) Staged sampling, determination of endogenous hormones in four developmental stages of pollen, the results show that the treatment of plant anther auxin content is always lower than the control, the average of only the control strain of 43.8% to 64.3%; abscisic acid content is always higher than The average content of zeatin was 1.33 ~ 1.87 times that of the control. The content of zeatin was always lower than that of the control, averaging only 39.6% ~ 57.1% of that of the control; the content of gibberellin was always lower than that of the control, averaging only 50.5% ~ 64.9%. (3) The content of amino acids in the anther of the treated and control plants was significantly lower than that of the control, the content of lysine, histidine, valine and methionine was significantly lower than that of the control, glutamic acid Content was significantly higher than the control. Based on the above results, it is inferred that the decrease of auxin, the increase of abscisic acid, the deficiency of cytokinin and gibberellin in anther are the basic biochemical factors leading to anther abortion. In addition, phenylalanine content was significantly lower, lysine, histidine, valine content was significantly lower and glutamate content was significantly higher, but also with cotton anther abortion to varying degrees.