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目的建立大鼠深低温停循环复苏模型。方法采用体外插管法建立大鼠闭胸式深低温停循环复苏模型,对其脑组织的病理形态学改变、超微结构的变化和动脉血气等各项指标进行检测。结果用大鼠建立深低温停循环复苏模型,呼吸的自主恢复率为80%,心跳恢复率为100%,同时对脑组织进行病理学观察,未见明显异常改变。结论大鼠深低温停循环复苏模型的建立,对研究脑组织低温状态下的病理生理学变化和临床应用低温进行脑保护提供了基本条件。
Objective To establish deep hypothermic circulatory arrest model in rats. Methods The closed-chest hypothermic circulatory arrest model was established by intubation in vitro. The pathological changes, ultrastructural changes and arterial blood gas of the rats were detected. Results The model of deep hypothermic circulatory arrest was established in rats. The spontaneous resuscitation rate was 80% and the rate of heart rate recovery was 100%. At the same time, the pathological changes of brain tissue were observed. Conclusion The establishment of deep hypothermic circulatory arrest model in rats provides the basic conditions for studying the pathophysiological changes at low temperature in brain tissue and clinical application of hypothermia for brain protection.