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稻瘟病是世界上危害最严重的水稻病害之一,鉴定与发掘抗稻瘟病种质资源是抗稻瘟病育种的重要基础工作。本研究利用与抗稻瘟病基因Pi1连锁的SSR引物MRG4766对173份云南地方稻种抗性基因Pi1进行了检测,并选择其中30个材料进行了人工接种验证。结果表明,人工接种验证与分子鉴定结果完全相符,说明SSR引物MRG4766用于鉴定抗稻瘟病基因Pi1是可行的。分子标记检测表明,供试的173份材料中有64份含有抗稻瘟病基因Pi1,占36.99%;其中102份籼稻中有33份含有该基因,占32.35%,而71份粳稻中有31份含有该基因,占43.66%,粳稻含有抗稻瘟病基因Pi1的比例比籼稻要高。检测到含有Pi1基因的种质材料分布在云南省11个州市29个县(市),分布范围较为广泛。南部边缘水陆稻区分布频率为41.03%,滇南单双季籼稻区分布频率为32.69%,滇中一季粳稻籼稻区分布频率为35.29%、滇东北高原粳稻区为35.29%、滇西北高原粳稻区33.33%。
Blast is one of the most damaging rice diseases in the world. Identification and discovery of germplasm resources resistant to rice blast is an important foundation for the breeding of blast resistance. In this study, we used 173 SSR primers MRG4766 linked to the blast resistance gene Pi1 to test Pi1 resistance gene in 173 Yunnan local rice varieties and selected 30 of them for artificial inoculation. The results showed that the artificial inoculation verification was in good agreement with the molecular identification results, indicating that the SSR primer MRG4766 could be used to identify the blast resistance gene Pi1. Molecular marker analysis showed that 64 of the 173 materials contained 36.1% of the blast resistance genes Pi1, of which 33 of 102 indica rice contained 32.35% of the total, and 31 of 71 japonica rice Containing the gene, accounting for 43.66%, japonica rice blast resistance gene Pi1 ratio is higher than indica rice. The germplasm materials containing Pi1 gene were detected in 29 counties (cities) in 11 prefectures and cities of Yunnan Province, with a wide range of distribution. The distribution frequency of paddy rice in the southern margin was 41.03%, that in Yunnan was 32.69% in single-and double-season indica rice, 35.29% in first-season indica rice in central Yunnan, 35.29% in japonica rice in northeastern Yunnan, 33.33%.