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本工作探讨血小板致密颗粒(dG)和α颗粒(αG)是否是细胞内钙库。在快速冷冻超薄切片上用电镜X射线显微分析法测定dG和αG的钙含量,分别为30.12±3.45和27.39±2.76 mmol/kg,证实含高钙。用磷钨酸乙醇(EPTA)染色的切片测得其钙含量分别为18.21±2.66和12.51±1.29 mmol/kg,说明钙有部分丢失。用激动剂A23187、凝血酶和ADP引起血小板变形时,两种颗粒的数密度明显下降,在EPTA染色切片上测出两种颗粒的钙进一步下降,下降幅度显著(P<0.01)。以上结果说明:1,血小板αG和dG皆含有高钙,是细胞内钙库,当分泌活动增强,颗粒数减少时,伴有颗粒钙释放;2,EPTA染色切片比冷冻切片的制备简便,适于颗粒钙含量变化的研究。
This work investigates whether platelet-dense granules (dG) and alpha granules (alphaG) are intracellular calcium stores. The calcium contents of dG and αG were determined by electron microscopy and X-ray microscopy on fast frozen ultrathin sections, which were 30.12 ± 3.45 and 27.39 ± 2.76 mmol / kg, respectively, confirming the high calcium content. Calcium contents measured by EPTA stained sections were 18.21 ± 2.66 and 12.51 ± 1.29 mmol / kg, respectively, indicating a partial loss of calcium. When platelet deformity was induced by agonists A23187, thrombin and ADP, the number densities of the two particles were significantly decreased. The calcium content of the two particles was further decreased by EPTA staining, with a significant decrease (P <0.01). The above results show that: 1, platelets αG and dG contain high calcium, is an intracellular calcium pool, when the secretory activity increased, the number of particles decreased, accompanied by the release of calcium particles; 2, EPTA staining sections than the frozen section preparation is simple, suitable Study on the Change of Particle Calcium Content.