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本文采用ODO高分辨扫描样品制备技术,观察了小白鼠、大白鼠和犬心肌纤维表面及断面的微细结构,并讨论了高分辨扫描电镜观察法的技术特点,分析了心肌纤维的超微结构及其与功能之间的关系。技术方法按常规取材、将样品修成梭形,放入1％锇酸中固定1～2小时;继以1/15M磷酸缓冲液清洗,用25％、50％的二甲基亚砜分别浸泡20分钟,并在TF-2型冷冻割断台上将样品割断;尔后,用1％锇酸对样品作后固定,0.1％锇酸软化细胞基质,再以1～2％单宁酸对样品作导电处理,用1％锇酸对样品进行终末固定;最后,对其进行脱水、干燥、镀膜处理后,用扫描电镜观察。
In this paper, ODO high resolution scanning sample preparation technology was used to observe the microstructure of the surface and the cross section of myocardial fibers in mice, rats and dogs. The technical characteristics of high resolution scanning electron microscopy were discussed. The ultrastructure of myocardial fibers and Its relationship with function. Technical methods according to conventional drawing, the sample was made into a spindle shape, placed in 1% osmium tetroxide fixed 1 to 2 hours; followed by 1 / 15M phosphate buffer washing, with 25%, 50% dimethylsulfoxide were soaked 20 Min, and the sample was cut off on a TF-2 freezing and cutting station; after that, the sample was post-fixed with 1% osmium tetroxide, 0.1% osmium tetroxide softened the cell substrate, and then the sample was subjected to 1 to 2% Treatment, with 1% osmium tetroxide on the sample terminal fixation; Finally, the dehydration, drying, coating treatment, the use of scanning electron microscopy.