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目的 :评价1 88Re标记抗CEA人 鼠嵌合抗体在人结肠癌裸鼠模型中的肿瘤靶向性。方法 :于裸鼠尾静脉注入1 88Re CEA人 鼠嵌合抗体后 2 4、4 8、72、96h分别测定荷瘤小鼠体内组织放射性分布 ,于不同时相分别对尾静脉注射1 88Re CEA嵌合抗体及1 88Re C5 0鼠单抗的裸鼠行放免显像。结果 :1 88Re CEA嵌合抗体注射后 9 5h ,肿瘤部位开始有放射性浓聚。以后随时间的增加 ,肿瘤部位放射性持续存在并维持较高水平 ,而周围组织放射性逐渐清除。1 88Re CEA人 鼠嵌合抗体与1 88Re C5 0抗体一样在肿瘤中有很高的摄取。肿瘤组织在注射1 88Re CEA人 鼠嵌合抗体 2 4h后 ,摄取放射性占总注入量的百分比为11 0 5 % ,随时间的延长稍有增加。 96h时所有脏器T NT比均大于 2 0。结论 :1 88Re CEA人 鼠嵌合抗体可特异地浓聚于结肠癌组织 ,有望用于临床诊断和治疗。
OBJECTIVE: To evaluate the tumor targeting of 1 88Re-labeled anti-CEA human chimeric antibody in a human colon cancer nude mouse model. METHODS: The radioactivity distributions of the tissues of the tumor-bearing mice were measured respectively at 24, 48, 72 and 96 hours after the mice were injected with the chimeric antibody of 1 88Re CEA in the tail vein of the nude mice. At the same time, Co-antibodies and 1 88Re C5 0 murine monoclonal antibody in nude mice. Results: 1 88Re CEA chimeric antibody injection 9 5h, tumor sites began to have radioactive accumulation. With the increase of time later, the radioactivity in the tumor site persisted and maintained at a high level, while radioactivity in the surrounding tissues gradually cleared. The 1 88 Re CEA human mouse chimeric antibody, like the 1 88 Re C5 0 antibody, had a high uptake in tumors. Tumor tissue after injection of 1 88Re CEA human chimeric antibody 2 4h, the percentage of total radioactive uptake was 11 0 5%, with the extension of time slightly increased. The T NT ratio of all organs at 96h was greater than 20. Conclusions: 1 88Re CEA human and mouse chimeric antibody can be specifically concentrated in colon cancer tissue, which is expected to be used in clinical diagnosis and treatment.