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目的探讨ATP敏感性钾(KATP)通道开放剂吡那地尔(Pin)对内皮素1(ET-1)诱导的人肺动脉平滑肌细胞(PASMCs)增殖及细胞内[Ca2+]i的影响。方法体外培养人PASMCs,用ET-1诱导其增殖,应用MTT法、Fluo-3和激光扫描共聚焦显微镜技术评价Pin对ET-1诱导的人PASMCs增殖及PASMCs[Ca2+]i调节的作用。结果 Pin显著抑制ET-1诱导的人PASMCs增殖,呈浓度依赖效应,KATP通道拮抗剂格列本脲呈浓度依赖性阻断Pin的作用;ET-1诱导人PASMCs内[Ca2+]i显著增加,Pin(10μmol/L)拮抗ET-1诱导的人PASMCs内[Ca2+]i升高。结论 KATP通道开放剂Pin可明显抑制ET-1诱导的人PASMCs增殖作用,抑制细胞内Ca2+浓度增加。
Objective To investigate the effects of Pinacidil, a KATP channel opener, on the proliferation and intracellular [Ca2 +] i of human pulmonary artery smooth muscle cells (PASMCs) induced by endothelin-1 (ET-1) Methods Human PASMCs were cultured in vitro and induced by ET-1. MTT assay, Fluo-3 and laser scanning confocal microscopy were used to evaluate the effects of Pin on ET-1-induced proliferation and PASMCs [Ca2 +] i regulation. Results Pin inhibited the proliferation of human PASMCs induced by ET-1 in a concentration-dependent manner. Glibenclamide (KATP channel antagonist) blocked Pin in a concentration-dependent manner. [Ca2 +] i in PASMCs induced by ET- Pin (10μmol / L) antagonized ET-1-induced increase of [Ca2 +] i in human PASMCs. Conclusions Pin, a KATP channel opener, can significantly inhibit the proliferation of human PASMCs induced by ET-1 and inhibit the increase of intracellular Ca2 + concentration.