论文部分内容阅读
用流式细胞术（ＦＣＭ）测定了４５例不同病期急性白血病患儿和１３例非白血病患儿骨髓细胞原位ＤＮＡ、ＲＮＡ和蛋白质含量，对急性淋巴细胞白血病（ＡＬＬ）治疗前、急性非淋巴细胞白血病（ＡＮＬＬ）治疗前、急性淋巴细胞白血病缓解期（ＣＲ）和非白血病患儿共四组病人的上述各项指标进行了分析。结果显示：１．急性白血病患儿的ＤＮＡ非整倍体检出率为４１．２％，其中ＡＬＬ为３３．３％，ＡＮＬＬ为６０％。ＡＬＬ和ＡＮＬＬ的Ｓ期细胞百分比（Ｓ％）显著低于非白血病患儿，而ＣＲ组的Ｓ％与非白血病的Ｓ％无显著性差异。２．ＡＮＬＬ组的ＲＮＡ含量显著高于ＣＲ组、非白血病组及ＡＬＬ组，而后三组间无显著性差异。３．四组之间的蛋白质含量无显著性差异。４．在对９例缓解期病人进行动态观察过程中，有５例检出ＤＮＡ非整倍体，其中４例在检出ＤＮＡ非整倍体后３３～７４天内复发。
The levels of DNA, RNA and protein in bone marrow of 45 children with acute leukemia at different stages and 13 non-leukemia children were determined by flow cytometry (FCM). Before acute lymphoblastic leukemia (ALL) The above indexes of four groups of patients before acute lymphoblastic leukemia (CR) and non-leukemia were analyzed before treatment of lymphocytic leukemia (ANLL). The result shows: 1. DNA aneuploidy detection rate of children with acute leukemia was 41.2%, ALL was 33.3%, ANLL was 60%. The percentage of S-phase cells (S%) in ALL and ANLL was significantly lower than that in non-leukemia children, while there was no significant difference in S% and non-leukemia S% of CR patients. 2. The RNA content of ANLL group was significantly higher than CR group, non-leukemia group and ALL group, but there was no significant difference between the latter three groups. 3. There was no significant difference in protein content among the four groups. 4. During the dynamic observation of 9 patients with remission stage, 5 cases detected DNA aneuploidy, of which 4 cases relapsed within 33 ~ 74 days after detecting DNA aneuploidy.