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目的检测S期激酶相关蛋白2(Skp2)和抑癌基因p27kip1在肾细胞癌(RCC)中的表达,探讨其与肾细胞癌生物学特征的关系及意义。方法采用组织芯片技术及免疫组化SP法检测Skp2和p27kip1在80例肾细胞癌组织和40例癌旁正常肾组织中的表达,并做统计学分析。结果(1)Skp2在肾细胞癌中的表达率高于正常肾组织(P=0.025);随肿瘤恶性程度增加,Skp2阳性表达率升高(P=0.002),其表达率与肾癌患者的年龄、性别、临床分期、淋巴结转移及肿瘤大小无关(P>0.05)。(2)p27kip1在肾细胞癌中的表达率低于正常肾组织(P=0.007);p27kip1阳性表达率与肿瘤分级及临床分期呈负相关((P<0.05),与肾癌患者的年龄、性别、淋巴结转移及肿瘤大小无关(P>0.05)。(3)Skp2与p27kip1表达呈显著负相关(r=-0.273,P=0.014)。结论肾细胞癌中Skp2蛋白的过度表达与p27kip1蛋白降解增强有关,提示Skp2可能在肾细胞癌发生中扮演着重要角色。
Objective To detect the expression of Skp2 and p27kip1 in renal cell carcinoma (RCC) and to explore its relationship with the biological characteristics of renal cell carcinoma. Methods The expression of Skp2 and p27kip1 in 80 cases of renal cell carcinoma and 40 cases of adjacent normal renal tissues were detected by tissue microarray technique and immunohistochemical SP method and analyzed statistically. Results (1) The expression of Skp2 in renal cell carcinoma was higher than that in normal renal tissues (P = 0.025). The positive expression of Skp2 was increased with the malignant degree (P = 0.002) Age, gender, clinical stage, lymph node metastasis and tumor size had no relation (P> 0.05). (2) The positive rate of p27kip1 in renal cell carcinoma was lower than that in normal renal tissues (P = 0.007). The positive rate of p27kip1 was negatively correlated with tumor grade and clinical stage (P <0.05) Gender, lymph node metastasis and tumor size (P> 0.05). (3) There was a significant negative correlation between Skp2 and p27kip1 expression (r = -0.273, P = 0.014) .Conclusion Skp2 overexpression in renal cell carcinoma is associated with p27kip1 protein degradation Enhanced, suggesting that Skp2 may play an important role in the occurrence of renal cell carcinoma.