论文部分内容阅读
目的：研究不同剂量ＤＦ－５２１（东菱克栓酶，Ｂａｔｒｏｘｏｂｉｎ）、低分子量肝素（ＬＭＷＨ）对体外培养人正常大隐静脉片内膜增生的影响。方法：体外培养人正常大隐静脉片，加入不同剂量ＤＦ－５２１（１０－４ＢＵ／ｍｌ，１０－２ＢＵ／ｍｌ）和低分子量肝素（０．１０８ＩＵ／ｍｌ，１０．８ＩＵ／ｍｌ）。培养最后４ｄ掺入５－溴脱氧尿核苷（５－ＢｒｄＵ）。应用免疫组织化学方法行抗５－ＢｒｄＵ单克隆抗体ＡＢＣ法染色及抗人平滑肌肌动蛋白（α－Ａｃｔｉｎ）单克隆抗体与Ｖｅｒｈｏｅｆ弹力纤维联合染色。并应用计算机图像分析仪进行图像分析，显微镜细胞计数等方法观察内膜增生及细胞增殖。结果：培养静脉片可见明显内膜增生和平滑肌细胞（ＳＭＣ）增殖。与对照组相比，较高浓度ＤＦ－５２１及低分子量肝素明显抑制培养血管片内膜增生。对照组内膜厚度（２７±３．４０）μｍ，ＬＭＷＨ（７±１．２０）μｍ，ＤＦ－５２１（６±１．２３）μｍ，Ｐ＜０．００１。对照组平滑肌细胞增殖指数（３０±２．２０）％，ＬＭＷＨ（６±１．２０）％，ＤＦ－５２１（５±１．７４）％，Ｐ＜０．００１。两种药物作用效果相近。结论：ＤＦ－５２１及低分子量肝素可抑制移植血管内膜增生及平滑肌?
Objective: To study the effects of different doses of DF-521 (Batroxobin) and LMWH on intima hyperplasia of human normal saphenous vein in vitro. Methods: Normal human saphenous vein was cultured in vitro. Different doses of DF-521 (10-4BU / ml, 10-2BU / ml) and low molecular weight heparin (0.108IU / ml, 10.8IU / ml) were added. The last 4 days of culture incorporates 5-bromodeoxyuridine (5-BrdU). Immunohistochemical staining was performed by ABC staining of anti-5-BrdU monoclonal antibody and anti-α-actin monoclonal antibody and Verhoef elastic fibers. The application of computer image analyzer for image analysis, microscopic cell counting and other methods to observe intimal hyperplasia and cell proliferation. Results: Intimal hyperplasia and smooth muscle cell (SMC) proliferation were observed in cultured venous. Compared with the control group, higher concentrations of DF-521 and low molecular weight heparin significantly inhibited intimal hyperplasia of cultured blood vessels. The thickness of the control group was 27 ± 3.40 μm, LMWH was 7 ± 1.20 μm, DF-521 was 6 ± 1.23 μm, P <0.001. The proliferation index of smooth muscle cells in control group (30 ± 2.20)%, LMWH (6 ± 1.20)% and DF-521 (5 ± 1.74)%, P <0.001. The effect of the two drugs are similar. Conclusion: DF-521 and low molecular weight heparin can inhibit vascular intimal hyperplasia and smooth muscle graft?