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人雄激素受体（ｈＡＲ）激素结合部（ＬＢＤ）的ｃＤＮＡ片段克隆到表达质粒ｐＴｒｘＡＲ内，在大肠杆菌ＧＩ７２４中可被诱导表达。通过改变诱导培养时间、诱导剂浓度、诱导温度及培养基的ｐＨ，在３４℃～３７℃，ｐＨ６．４～７．７的培养基中，用１００μｇ／ｍｌＴｒｐ诱导培养４ｈ，可获得高效表达的ＬＢＤ融合蛋白产物。其最高表达量为５０～７０ｍｇ／Ｌ湿菌体。产物主要以包含体形式存在。经尿素溶解后，由ＳｅｐｈａｃｒｙｌＳ－２００柱层析获得了电泳纯的目的产物。氨基酸组分分析和相对分子量测定表明与理论值一致。该表达条件的确定，为ｈＡＲ的ＬＢＤ基因表达和进一步研究提供了依据。
The cDNA fragment of human androgen receptor (hAR) hormone binding part (LBD) was cloned into the expression plasmid pTrxAR and expressed in E. coli GI724. By changing the induction culture time, inducer concentration, induction temperature and pH of the medium, cultured in medium of 34 ℃ ~ 37 ℃, pH 6.4 ~ 7.7 with 100μg / ml Trp for 4h, LBD fusion protein product. The highest expression of 50 ~ 70mg / L wet cells. The product is mainly in the form of inclusion bodies. After urea dissolution, the pure product of electrophoresis was obtained by Sephacryl S-200 column chromatography. Amino acid component analysis and relative molecular weight determination showed consistent with the theoretical value. The determination of the expression conditions provided the basis for the expression of LBD gene of hAR and further study.