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目的研究1,6-二磷酸果糖(FDP)在对抗自由基、细胞因子及维护肺脏组织结构方面对自体肺体外循环中肺的保护作用。方法将12只健康小猪随机分为2组,每组6只:对照组为自体肺体外循环组;实验组将FDP加入体外循环预充液中。两组体外循环均持续2h,于体外循环停机后检测肺组织肿瘤坏死因子-α、肺组织丙二醛的含量并观察术后两组肺组织的病理学变化。结果体外循环后实验组肺组织TNF-α与丙二醛含量明显低于对照组(P<0.05),肺组织标本的光镜观察显示:实验组肺毛细血管淤血程度,中性粒细胞及淋巴细胞聚集程度,肺组织损伤程度均较对照组显著减轻。结论 1,6-二磷酸果糖能明显降低自体肺体外循环术后肺组织自由基和TNF-α的含量,减轻肺组织损伤,对肺组织有明显的保护作用。
Objective To study the protective effects of fructose-1,6-diphosphate (FDP) on the lungs during autologous pulmonary extracorporeal circulation against free radicals, cytokines and the maintenance of lung tissue structure. Methods Twelve healthy piglets were randomly divided into 2 groups with 6 rats in each group. The control group was autologous extracorporeal circulation group. In the experimental group, FDP was added into the extracorporeal circulation priming solution. Both of the two groups continued for 2 hours after cardiopulmonary bypass. The levels of tumor necrosis factor-α and malondialdehyde in lung tissue were measured after cardiopulmonary bypass and the pathological changes of lung tissue in both groups were observed. Results The levels of TNF-α and malondialdehyde in lung tissue of experimental group after CPB were significantly lower than those of control group (P <0.05). The light microscope observation of lung tissue showed that the degree of pulmonary capillary congestion, neutrophil and lymph The degree of cell aggregation and lung injury were significantly reduced compared with the control group. Conclusion Fructose-1,6-diphosphate can significantly reduce the content of free radicals and TNF-α in the lung tissue after autologous extracorporeal circulation and reduce the damage of lung tissue, which has a significant protective effect on lung tissue.