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Objective:To study the effect of maternal BDE-209 (brominated Diphenyl Ethers-209)exposure on the expression of microtubule-associated protein-1b (map-1b) and S-100 in rat’s hippocampus of the offspring by RT-PCR.Methods:Peanut oil suspensions of commercial deca-BDE was given in dose of 300 mg/(kg·d) by oral gavage throughout gestation and lactation in experimental group.The control group was administered only with the same capacity of peanut oil at the same time.The expression of MAP-1B in the hippocampus of the offspring’s rats were tested when the pups were newborn,7days,14 days,21days and 45days old respectively by means of RT-PCR.Result:MAP-1B protein showed a statistically significantly lower concentration in the groups 14 days,21days,45days than that of the control groups.The expression of S-100 in the group which received with deca-BDE by RT-PCR showed higher than that of control groups.But only the 45days groups had significant difference of expression of MAP-1B protein compared with the control groups(P<0.05).Conclusions:Maternal BDE-209 exposure during the period of pregnancy will diminish the expression of map-1b protein in hippocampus of offspring’s rats.
Objective: To study the effect of maternal BDE-209 exposure on the expression of microtubule-associated protein-1b (map-1b) and S-100 in rat’s hippocampus of the offspring by RT-PCR. Methods : Peanut oil suspensions of commercial deca-BDE was given in dose of 300 mg / (kg · d) by oral gavage throughout gestation and lactation in experimental group. The control group was administered only with the same capacity of peanut oil at the same time MAP-1B protein showed a significant significant lower in the hippocampus of the offspring’s rats were tested when the pups were newborn, 7 days, 14 days, 21 days and 45 days old by means of RT-PCR. concentration in the groups 14 days, 21days, 45days than that of the control groups. The expression of S-100 in the group which received with deca-BDE by RT-PCR showed higher than that of control groups. Only the 45days groups had significant difference of expression of MAP-1B protein compa red with the control groups (P <0.05) .Conclusions: Maternal BDE-209 exposure during the period of pregnancy will diminish the expression of map-1b protein in hippocampus of offspring’s rats.