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Objective:To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction(清瘟败毒饮,QBD) from the view of holistic biology.Methods:The rats were randomly divided into a normal conrol group,a lipopolysaccharide(LPS) group,the low- and high-dose QBD groups,and a dexamethasone(DXM) group.NR8383 cells were treated with culture fluid containing 6%serum from rats of each group respectively.Inflammatory mediators were detected by reverse transcription polymerase chain reaction(RT-PCR),Western blotting hybridization,enzyme linked immunosorbent assay(ELISA),polymerase chain reaction(PCR) gene array and antibody array.Results:It is showed that the levels of interleukin(IL)-1 α,IL-4 and IL-12 were enhanced in the low-dose QBD group;levels of IL-1 α,IL-12 and IL-18 were augmented in the high-dose QBD group,compared with the LPS group after ELISA detection.Western blot showed that IL-1β and tumor necrosis factor(TNF)-α expression of the control group were lower than other groups.IL-1 βlevel of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after24 h than that of the control group(P<0.01).Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array.Up-regulation genes were 22 in both the LPS group and the low-dose QBD group,among which 16 up-regulating genes were the same.In these 16 genes,the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group,4 were similar to and 3 were more.Twenty-nine main cytokines were inspected by rat cytokine antibody array.Intergroup gray value differences were found in 7 expressed cytokines.The levels of these 7 cytokines in the lowdose QBD group were all lower than those in the the LPS group.Conclusions:QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.
Objective: To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction from the view of holistic biology. Methods: The rats were randomly divided into a normal conrol group, a lipopolysaccharide (LPS) group, the low and high-dose QBD groups, and a dexamethasone (DXM) group. NR8383 cells were treated with culture fluid containing 6% serum from rats of each group respectively. Inflammatory mediators were detected by reverse transcription polymerase chain reaction (RT -PCR), Western blotting hybridization, enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) gene array and antibody array. Results: It was showed that the levels of interleukin (IL) -1 α, IL-4 and IL -12 were enhanced in the low-dose QBD group; levels of IL-1 α, IL-12 and IL-18 were augmented in the high-dose QBD group, compared with the LPS group after ELISA detection. Western blot showed that IL -1β and tumor necrosis factor (TNF) -α expression of the control group were lower than other groups. IL-1 βlevel of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after 24 h than that of the control group (P <0.01). Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array. Up-regulation genes were 22 in both the LPS group and the low-dose QBD group, among which 16 up-regulating genes were the same. These 16 genes , the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group, 4 were similar to and 3 were more.Twenty-nine main cytokines were inspected by rat cytokine antibody array.Intergroup gray value differences were found in 7 expressed cytokines. these levels of these 7 cytokines in the lowdose QBD group were all lower than those in the the LPS group. Conclusions: QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.