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潜育性水稻田广泛分布于中国、斯里兰卡、印度、印度尼西亚、塞拉里昂、利比亚、尼日利亚、哥伦比亚和菲律宾等国 ,其中我国南方稻区就有 6 70万公顷低产潜育性水稻田。该类水稻田还原性强 ,矿质营养失调 ,尤以Fe2 + 过量积累 ,对水稻生长发育产生不良的逆境胁迫作用。培育抗亚铁毒的水稻品种是简便、经济有效地提高稻谷产量的重要途径之一。本文利用Kinmaze DV85的 81个重组自交家系 (RILs)的作图群体 ,采用水培鉴定方法 ,在亚铁毒条件下 ,检测叶片棕色斑点指数 (LBI)、株高 (PH)的数量性状位点 (QTLs)。结果表明 ,控制叶片棕色斑点指数、株高的数量性状位点都位于第3染色体上 ,各QTL的LOD值为 3.79～ 5 .89。检测到与亚铁毒胁迫直接有关的性状叶片棕色斑点指数QTL 2个 ,分别位于第 3染色体的X2 79 C2 5和X1 4 4 X36 2间 ,对应的贡献率分别为 1 7.38%和 2 2 .0 7% ,其中位于第 3染色体X2 79 C2 5间的叶片棕色斑点指数QTL与水稻功能图谱第 3染色体上的控制叶绿素含量的QTL位置一致 ;另一个位于X1 4 4 X36 2间的叶片棕色斑点指数QTL与水稻功能图谱第 3染色体上的另一个控制叶绿素含量的QTL连锁 ,相距 2 5 .6cM。表明在亚铁毒胁迫条件下 ,水稻在其叶片表面出现棕色斑点 ,叶片衰老 ,产生一些叶绿素降解物
Drought paddy fields are widely distributed in China, Sri Lanka, India, Indonesia, Sierra Leone, Libya, Nigeria, Colombia and the Philippines. Among them, 6.7 million hectares of low-yielding and fertile paddy fields are found in southern China. This type of paddy rice is highly reductive and has a mineral nutrient imbalance, especially excessive accumulation of Fe2 +, which has adverse adverse stress effects on the growth and development of rice. Breeding anti-ferrous iron rice varieties is an easy, economical and effective way to improve rice production is one of the important ways. In this study, 81 maize inbred lines (RILs) from Kinmaze DV85 were used for mapping population. The hydroponics method was used to detect the leaf brown speckle index (LBI) and quantitative characters of plant height (PH) Points (QTLs). The results showed that the quantitative trait loci of brown leaf spot index and plant height were all located on chromosome 3, and the LOD value of each QTL was 3.79-5.89. Two QTLs for brown spot index of leaf traits directly related to ferrous stress were detected, which were located on chromosomes X2 79 C2 5 and X1 4 4 X36 2, respectively, corresponding to 1 7.38% and 2 2, respectively. 0 7%. The QTL for leaf brown speckle index located on chromosome X2 79 C2 5 was consistent with the QTL controlling chlorophyll content on chromosome 3 of rice functional map. The other leaf spot brown spot on X1 4 4 X36 2 The index QTL was linked to another QTL controlling chlorophyll content on chromosome 3 of rice map, which was 25.6 cM. The results showed that under the condition of ferrous iron stress, brown spots appeared on the surface of rice leaves and the leaves became senescent to produce some chlorophyll degradation products