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用“格兰尼、史密斯”(Granny Smith)苹果的腋芽离体培养产生了茎芽。这种茎芽采用标准的繁殖方法,难以从离体培养中诱导不定根的形成。在静置的液体培养下没有形成根,而在琼脂为基础的培养基或带有滤纸桥的培养基上生根也不多(约4％)。当生长在连续振动液体培养基上(1/2MS培养基)以及在连续光照和恒温(26℃±2℃)下,茎条生根率可高达80％。外源生长素对生根是不可少的。吲哚乙酸(IBA)10μM或萘氧乙酸(NOA)10μM都促进了根的形成,但是2.4-D则抑制根的形成。蔗糖的最适浓度为1％(W/V)。根的创伤增进了根的发生。不定根的起源是随施用的生长素而不同:用萘氧乙酸是从切面生根,用吲哚丁酸,根是从表皮层和从切面都可长出。
In vitro culture of axillary buds from Granny Smith apples resulted in stem buds. This stem shoot uses standard breeding methods and is difficult to induce adventitious root formation from in vitro culture. No roots formed under standing liquid culture, whereas rooting was less (about 4%) on agar-based medium or media with filter paper bridges. The rooting rate of stems can be as high as 80% when grown on continuous vibratory broth (1/2 MS medium) and under continuous light and constant temperature (26 ° C ± 2 ° C). Exogenous auxin is essential for rooting. Indoleacetic acid (IBA) 10 μM or Naphthyoxyacetic acid (NOA) 10 μM promoted root formation but 2.4-D inhibited root formation. The optimum concentration of sucrose is 1% (W / V). Root trauma promotes root development. The origin of adventitious roots is different depending on the auxin to be used: Naphthyoxyacetic acid is rooted from the cut surface with indole butyric acid, and the roots grow from the epidermis and from the cut surface.