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目的 :观察三氧化二砷 (ArsenicTrioxide ,As2 O3 )对大肠癌LoVo细胞的体外杀伤作用 ,并研究其和 5 氟脲嘧啶(5 FU)的协同作用。方法 :用MTT法检测细胞的体外生长抑制情况。用光镜、电镜观察细胞的形态学变化 ,流式细胞仪检测细胞周期的变化。结果 :As2 O3 对LoVo细胞的抑制作用随着浓度和作用时间的增加而增大 ,通过光镜、电镜可观察到细胞的凋亡。经流式细胞仪检测As2 O3 作用后的肿瘤细胞 ,G0 /G1细胞从 42 3%下降到 31 3%,S期细胞从 39%上升到 6 2 %。药物作用48h ,As2 O3 (0 3mg/L)组抑制率为 30 38%,而联合治疗组抑制率增至 73 75 %。结论 :As2 O3 对LoVo细胞具有杀伤作用 ,其对LoVo细胞生长抑制的影响主要作用于G0 /G1期细胞。As2 O3 与 5 FU对大肠癌LoVo细胞具有协同作用。
Objective: To observe the killing effect of Arsenic Trioxide (As 2 O 3) on LoVo cells in vitro and the synergistic effect with 5-fluorouracil (5 FU). Methods: MTT assay was used to detect the growth inhibition of cells in vitro. Morphological changes of cells were observed with light microscope and electron microscope, and the changes of cell cycle were detected by flow cytometry. Results: The inhibitory effect of As2 O3 on LoVo cells increased with the increase of concentration and time. The apoptosis of LoVo cells was observed by light and electron microscopy. As2 O3-treated tumor cells were detected by flow cytometry. The percentage of G0 / G1 cells decreased from 42 3% to 31 3%, while that of S phase increased from 39% to 62%. The inhibition rate of As2O3 (0 3mg / L) group was 30 38% at 48h, while that of combined treatment group was 73 75%. CONCLUSION: As2 O3 has cytotoxic effect on LoVo cells, and its effect on growth inhibition of LoVo cells mainly affects G0 / G1 phase cells. As2 O3 and 5 FU have a synergistic effect on colorectal cancer LoVo cells.