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目的建立评价茵栀黄注射液质量的指纹图谱分析方法。方法高效液相色谱条件Kromasil 100-5C_(18)色谱柱(4.6 mm×25 cm,5μm);甲醇-0.05%磷酸溶液为流动相进行二元梯度洗脱;流速1.0 mL·min~(-1);检测波长为238nm;柱温30℃。结果建立了茵栀黄注射液中绿原酸类、栀子苷类、黄芩苷类成分的指纹图谱,并建立了共有峰模式、全谱相似度和去黄芩苷相似度模式3种指纹图谱分析评价方法。结论建立的茵栀黄注射液指纹图谱的重现性、稳定性好,可以有效地控制茵栀黄注射液的质量。
Objective To establish a fingerprint analysis method for evaluating the quality of Yinzhihuang injection. Methods High performance liquid chromatography (HPLC) was performed on a Kromasil 100-5C 18 column (4.6 mm × 25 cm, 5 μm) with a mobile phase of methanol-0.05% phosphoric acid. The flow rate was 1.0 mL · min -1 ); Detection wavelength of 238nm; column temperature 30 ℃. Results Fingerprints of chlorogenic acid, geniposide and baicalin in Yinzhihuang injection were established, and three fingerprints of common peak pattern, full spectrum similarity and similarity to baicalin were established Evaluation method. Conclusion Yin Zhi Huang injection fingerprint established reproducibility, good stability, can effectively control the quality of Yin Zhi Huang injection.