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赫金格尔培养基冷至50℃时,按每9 ml培养基内加入DNA溶液1 ml和10%氯化钙溶液0.1 ml,混匀后分装试管,每管10 ml,于0.5大气压灭菌20分钟,可在4℃保存6个月以上。DNA溶液的配制:取DNA 1 g,溶于100 ml蒸馏水中,加0.8%NaOH溶液5ml,混匀后于水浴内加热溶解。测定时,将溶化并冷至45℃的上述试管内培养基,倾注于无菌平皿内。培养基表面点状接种经24小时培养的葡萄球菌(接种环直径0.2 mm),每一平皿接种不超过25份培养物,37℃培养24小时,然后在平皿表面注入
Hegengel medium cold to 50 ℃, according to 9 ml of culture medium by adding 1 ml of DNA solution and 10% calcium chloride solution 0.1 ml, after mixing aliquot test tube, each tube 10 ml, at 0.5 atmospheric pressure off Strain 20 minutes, can be stored at 4 ℃ for 6 months or more. Preparation of DNA solution: Take DNA 1 g, dissolved in 100 ml of distilled water, add 5% 0.8% NaOH solution, mix well and dissolve in water bath. In the assay, the above in vitro culture medium, which melted and cooled to 45 ° C, was poured into a sterile dish. Staphylococcus aureus (0.2 mm diameter inoculation loop) was inoculated on the surface of the medium in dotted fashion for 24 hours. No more than 25 cultures were inoculated into each plate and incubated at 37 ° C for 24 hours before being injected on the surface of the plate