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目的:探讨核转录因子KLF7对脱细胞异体神经支架(ANA)移植修复坐骨神经缺损后小鼠运动轴突再生和运动功能恢复的影响。方法:制作C57BL/6小鼠坐骨神经缺损模型,用ANA进行桥接修复,在ANA注射腺相关病毒2(AA2)或腺相关病毒2-KLF-7(AAV2-KLF7,2μl,1×10~(11)病毒颗粒)。于修复术后4周,Western Blot和免疫荧光染色检测ANA内KLF7蛋白表达,NF和S100免疫荧光染色检测ANA内轴突再生和髓鞘生成,神经示踪剂荧光金(FG)逆行标记检测运动轴突再生,坐骨神经运动功能指数(SFI)和电生理检测运动功能的恢复。结果:AAV2-KLF7注射ANA后4周,ANA内KLF7蛋白表达显著增高,NF和S100表达显著增加,FG阳性标记脊髓运动神经元的数量增加,坐骨神经功能指数增加,电生理动作电位波幅增大,潜伏期缩短(P<0.05)。结论:KLF7促进小鼠脱细胞异体神经支架修复坐骨神经缺损后运动轴突再生和髓鞘形成,有助于运动功能恢复。
OBJECTIVE: To investigate the effect of KLF7 on axonal regeneration and motor function recovery of axonal cells after allogeneic neural scaffold (ANA) transplantation in repairing sciatic nerve defects. METHODS: A model of sciatic nerve defect was established in C57BL / 6 mice. ANA was used to repair the sciatic nerve defects. ANA was injected with adeno-associated virus 2 (AA2) or adeno-associated virus 2-KLF- ) Virus particles). At 4 weeks after repair, KLF7 protein expression in ANA was detected by Western Blot and immunofluorescence staining, axon regeneration and myelination in ANA were detected by NF and S100 immunofluorescence staining, and retrograde labeling with fluorescent neurotrophic factor (FG) Axonal regeneration, sciatic motor function index (SFI) and electrophysiological recovery of motor function. RESULTS: After AAV2-KLF7 injection for 4 weeks, the expression of KLF7 protein in ANA was significantly increased, while the expression of NF and S100 was significantly increased. The number of FG-positive spinal cord motoneurons increased, the sciatic nerve function index increased, the amplitude of electrophysiological action potentials increased, Latency decreased (P <0.05). CONCLUSION: KLF7 can promote the regeneration of motor axons and myelination after repairing sciatic nerve defects by acellular allogeneic nerve scaffold in mice, which is helpful for recovery of motor function.