论文部分内容阅读
目的探索艾滋病病毒(HIV)阳性的静脉吸毒(IDU)人群和男男性行为(MSM)人群,IgG、IgG1-4是否有差异。方法抽取652份血清/血浆样品,其中正常人315份,HIV阳性静脉吸毒者222份,HIV阳性男男性行为者115份。HIV阳性血浆根据CD4+T淋巴细胞数量分为两组:<350细胞/μl组和≥350细胞/μl组;并且用夹心酶联免疫吸附试验(ELISA)定量检测IgG水平,用竞争ELISA方法检测IgG1-4水平。结果 HIV阳性IDU人群:其IgG与健康人群相比无差异,其IgG1和IgG2低于健康人群,IgG3和IgG4高于健康人群;HIV阳性MSM人群:其IgG、IgG1和IgG3高于健康人群,但CD4+≥350μl组的IgG和IgG1与健康人群相比无差异,IgG2低于健康人群,IgG4则是CD4<350/μl组与健康人群相比无差异,而CD4+≥350/μl组高于健康人群。结论 HIV阳性IDU人群和MSM人群之间IgG、IgG1和IgG4的变化趋势有差异,IgG2和IgG3的变化趋势相同。
Objective To explore the differences of IgG and IgG1-4 between HIV positive IDU population and MSM population. Methods A total of 652 serum / plasma samples were collected, including 315 normal subjects, 222 HIV-positive intravenous drug users and 115 HIV-positive MSM. HIV-positive plasma was divided into two groups according to the number of CD4 + T lymphocytes: <350 cells / μl and ≧ 350 cells / μl; and the IgG level was quantitatively determined by a sandwich enzyme-linked immunosorbent assay (ELISA) IgG1-4 level. Results There was no difference in HIV-positive IDU population: the IgG1 and IgG2 were lower than those in the healthy population, IgG3 and IgG4 were higher than those in the healthy population; the HIV-positive MSM population was higher in the IgG, IgG1 and IgG3 than in the healthy population CD4 +> 350μl group IgG and IgG1 no difference compared with healthy people, IgG2 lower than healthy people, IgG4 CD4 <350 / l group and no difference compared with healthy people, CD4 +> 350 / l group than healthy people . Conclusion The trend of IgG, IgG1 and IgG4 between HIV positive IDU population and MSM population is different, and the trend of IgG2 and IgG3 are the same.