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作者将制备视网膜毛细血管平铺标本的胰蛋白酶消化技术加以改进以便与脑组织毛细血管比较,其方法如下:将福尔马林固定的脑皮质切成小块(1×1×0.3cm)并剥去软脑膜。水冼和递增浓度酒精脱水。在甲醛、氯仿混合液中进一步脱脂,在24小时内不断摇动。然后经递减浓度的酒精回到水中,用3％胰蛋白酶缓冲液消化1～3小时。在侧照光和暗背景下观察血管碎片,将其置于载片,选用PAS和苏木精染色。结缔组织和某些其它组织的毛细血管壁细胞位于血管壁外,而脑部血管象视网膜一样,位于神经—胶质中且有同样的厚壁毛细血管。视网膜与脑皮质血管的相同点:
The authors modified trypsin digestion techniques to prepare retinal capillary tibial specimens for comparison with brain tissue capillaries by cutting the formalin-fixed cerebral cortex into small pieces (1 x 1 x 0.3 cm) Peel the pial. Water and increasing concentrations of alcohol dehydration. In formaldehyde, chloroform mixture further degreasing, shaking constantly within 24 hours. Then returned to water with decreasing concentrations of alcohol and digested with 3% trypsin for 1 to 3 hours. Blood vessels were observed under side-light and dark background, placed on slides and stained with PAS and hematoxylin. The capillary wall cells of connective tissue and some other tissues are located outside the walls of the blood vessels, and the blood vessels of the brain, like the retina, are located in the neuro-glia with the same thick-walled capillaries. Retina and cerebral cortex vascular similarities: