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大豆原生质体培养诱导再生植株一直为国内外学者所关注。如Kao(1970)和Miller等(1971)从悬浮培养的大豆细胞分离出原生质体,经培养获得了愈伤组织。但在以后的多年中进展不够大。据报道,已从幼荚子叶、幼苗根、叶肉组织和悬浮培养细胞等外植体游离出原生质体,经培养获得愈伤组织,并进行了大量的分化研究,但最终都未能得到再生植株。卫志明和许智宏于1988年首次由大豆幼荚子叶分离出
Soybean protoplast-induced regeneration plants have been of concern to domestic and foreign scholars. Protoplasts were isolated from suspension-cultured soybean cells as described by Kao (1970) and Miller et al. (1971), and callus was obtained by culturing. However, progress has not been enough in many years to come. It has been reported that protoplasts have been released from explants such as young pod cotyledon, seedling root, mesophyll tissue and suspension cultured cells, callus obtained by culturing and extensively differentiated, but ultimately failed to obtain regenerated plants . Wei Zhiming and Xu Zhihong in 1988 for the first time separated from the soybean young Podocarpus leaves