论文部分内容阅读
目的：探讨高速枪弹伤时产生的强压力波对血管内皮细胞内皮素（ＥＴ－１）的释放及基因表达的影响。方法：以高速枪弹致伤犬腹部以及高速枪弹压力波致伤培养人脐静脉内皮细胞为实验模型，采用放射免疫及斑点杂交的方法，从整体、细胞、基因不同层次观察内皮细胞ＥＴ－１释放及表达的特点。结果：致伤犬和培养的内皮细胞在压力波作用下，ＥＴ－１释放和基因表达的变化具有相似的趋势，出现即刻和延迟反应，即伤后１０分钟至６小时ＥＴ－１释放明显增加，ｍＲＮＡ（ＥＴ－１）转录增强则出现在伤后４～６小时。结论：压力波是导致ＥＴ－１释放及基因表达紊乱的重要力学因素，ＥＴ－１ｍＲＮＡ转录增强可能是压力波对ＥＴ－１调节的延迟效应的分子病理学基础。
OBJECTIVE: To investigate the effect of intense pressure wave generated by high-speed gunshot wound on the release and gene expression of endothelin (ET-1) in vascular endothelial cells. Methods: Human umbilical vein endothelial cells (HUVECs) were induced by high-speed bullet-induced canine abdominal injury and high-speed bullet pressure wave injury. ET-1 release from endothelial cells was observed by radioimmunity and dot blot hybridization And the characteristics of expression. Results: The changes of ET-1 release and gene expression in wounding dogs and cultured endothelial cells showed a similar trend under pressure wave, with immediate and delayed reactions, ie, the ET-1 release significantly increased from 10 minutes to 6 hours after injury , MRNA (ET-1) increased transcription appeared in 4 to 6 hours after injury. CONCLUSION: Pressure wave is an important mechanical factor leading to ET-1 release and gene expression disturbance. The enhanced transcription of ET-1 mRNA may be the molecular pathological basis of the delayed effect of pressure wave on ET-1 regulation.