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我们在舟山白泉地区阿米巴流行病学调查时,对2294份粪便标本中所查获的140份溶组织内阿米巴包囊,进行了测量观察,初步分出“大型”与“小型”。现报告如下。 一、方法与结果 1.取新鲜粪便标本(1~3小时以内),以生理盐水直接涂片法检查阿米巴(其中有38份包囊,系同时用醛醚沉淀与3次换水自行沉淀法检查获得)。所检出之溶组织内阿米巴包囊标本,作卢戈氏碘液染色后测量观察。 2.接目镜(10×)内安放目镜测微计,在高倍视野下(放大650倍)测量观察,记录结果。 3.分型标准:以包囊平均直径10μm为界限,划分“大型”与“小型”。每份标本测量包囊5~10
In the epidemiological investigation of amoeba in Baiquan area, Zhoushan, we surveyed 140 cases of Entamoeba histolytica encystoma collected from 2294 stool specimens and observed the “large” and “small”. The report is as follows. First, methods and results 1. Take fresh stool specimens (1 to 3 hours), saline direct smear method to check amoeba (of which 38 were cysts, Department of aldehyde ether precipitation simultaneously with three water changes on their own Precipitation method). The detected E. histolytica cysts specimens for Lugol’s iodine staining after the observation. 2 eyepiece (10 ×) placed eyepiece micrometer, high magnification field of view (magnification 650 times) to observe the measurement, record the results. 3. Classification criteria: the average diameter of 10μm capsule as a boundary, divided into “large” and “small.” Each specimen measured cysts 5 to 10