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目的设计新型结构磁性脂质体,并通过自组装法制备。方法采用共沉淀法制备Fe3O4磁性纳米粒子,采用薄膜分散法制备多柔比星脂质体,测定Fe3O4粒子和多柔比星脂质体的Zeta电位,根据电荷性质选择适当的聚电解质,依次将聚电解质和Fe3O4粒子组装到多柔比星脂质体表面,得到磁性脂质体,并采用透射电镜(TEM)观察其形貌。结果 Fe3O4粒子和多柔比星脂质体的Zeta电位分别为-22.0、-22.6 mV,自组装过程中选择阳离子聚电解质PDADMAC作为中间层,磁性脂质体的TEM照片显示,其具有较规则的球形轮廓,表面均匀吸附有大量的Fe3O4磁性粒子使得脂质体表面略显粗糙,证实了新型结构磁性脂质体的成功构建。结论采用静电自组装法可以制备新型结构磁性脂质体。
Objective To design novel magnetic liposomes and prepare them by self-assembly method. Methods Fe3O4 magnetic nanoparticles were prepared by co-precipitation method, and the doxorubicin liposomes were prepared by thin-film dispersion method. The Zeta potential of Fe3O4 particles and doxorubicin liposomes were determined. The appropriate polyelectrolytes were selected according to the charge properties. The polyelectrolyte and Fe3O4 particles were assembled on the surface of doxorubicin liposomes, and magnetic liposomes were obtained. The morphologies of the magnetic liposomes were observed by transmission electron microscopy (TEM). Results The Zeta potentials of Fe3O4 particles and doxorubicin liposomes were -22.0 and -22.6 mV, respectively. The cationic polyelectrolyte PDADMAC was selected as the intermediate layer during self-assembly. The TEM images of the magnetic liposomes showed that the Zeta potential of the particles was more regular Spherical profile, the surface of the uniform adsorption of a large number of Fe3O4 magnetic particles make the liposome surface slightly rough, confirmed the successful construction of a new type of magnetic liposomes. Conclusion The new structure of magnetic liposomes can be prepared by electrostatic self-assembly method.