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利用基因枪轰击水稻未成熟胚及未成熟胚诱导来源的愈伤组织，对国内外16个籼稻品种进行了转化。设置不同的基因枪转化参数，并在培养基中添加各种植物激素、甘露醇以及2－N－吗啉－乙基磺酸（简称MES）来改善继代过程中籼稻愈伤组织的生长状态。基因枪转化的操作参数（包括金粉浓度和DNA含量、基因枪氦气压力、轰击受体的不同状态）对转化频率有重要影响；在继代培养基中添加MES（500mg／L）作为缓冲剂，可以减少籼稻愈伤组织褐化现象的发生；在诱导和继代培养基中补加NAA（0．5mg／L），KT（0．2mg／L）可改进愈伤组织质量，进而提高再生频率；在继代培养基中补加低浓度的ABA（1mg／L）和甘露醇（20g／L），能普遍改善愈伤组织生长状况，减少褐化现象的产生，得到较多结构致密的颗粒状抗性愈伤。获得了再生的转化植株，经检测表现出对Basta的抗性。
Sixteen indica rice varieties at home and abroad were transformed using gene gun bombardment of callus induced from immature and immature embryos of rice. Different gene gun transformation parameters were set up and various plant hormones, mannitol and 2-N-morpholino-ethyl sulfonic acid (MES) were added to the medium to improve the growth status of indica rice during the subculture . The operating parameters of the biolistic transformation (including the concentration of the gold powder and the DNA content, the gene gun helium pressure, different states of the bombardment receptor) have an important effect on the transformation frequency; MES (500 mg / L) is added as a buffer in the subculture medium , Can reduce the browning of indica rice callus; addition of NAA (0.5mg / L) and KT (0.2mg / L) in the induction and subculture medium can improve callus quality, and then improve regeneration The addition of low concentrations of ABA (1 mg / L) and mannitol (20 g / L) to the subculture medium can generally improve the callus growth and reduce browning, resulting in more dense structure Granular resistance to callus. Regenerated transformed plants were obtained and tested to show resistance to Basta.