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背景与目的:观察桑黄胞内多糖(PLIP)对体外培养的人白血病细胞K562的增殖抑制作用,并初步探讨其作用机制。材料和方法:采用MTT法及台盼蓝拒染法测定细胞增殖抑制率和生长曲线,Hoechst_PI双荧光染色,流式细胞术和DNA琼脂糖凝胶电泳检测细胞凋亡。结果:PLIP在25μg/ml、50μg/ml、100μg/ml、200μg/ml、400μg/ml、800μg/ml剂量时均能显著抑制K562细胞增殖(P<0.05),其中400μg/ml剂量时抑制率最高,达52.55%,半数抑制浓度(IC50)为262.36μg/ml,流式细胞仪检测PLIP在100μg/ml、200μg/ml、400μg/ml剂量时K562细胞凋亡率分别为5.72%、13.57%、19.39%,并能诱导K562细胞出现凋亡所具有的形态学及生化特征。结论:PLIP对体外培养的人白血病细胞K562生长增殖具有明显的抑制作用,其作用机制可能与诱导K562细胞凋亡和影响细胞周期有关。
BACKGROUND & AIM: To observe the inhibitory effect of PLIP on proliferation of human leukemia cell line K562 cultured in vitro and to explore its mechanism. MATERIALS AND METHODS: Cell proliferation inhibition rate and growth curve were determined by MTT method and trypan blue exclusion method. Cell apoptosis was detected by double staining with Hoechst_PI, flow cytometry and DNA agarose gel electrophoresis. Results: PLIP significantly inhibited the proliferation of K562 cells (P <0.05) at doses of 25μg / ml, 50μg / ml, 100μg / ml, 200μg / ml, 400μg / ml and 800μg / And the IC50 was 262.36μg / ml. The apoptotic rates of K562 cells treated with PLIP at 100μg / ml, 200μg / ml and 400μg / ml by flow cytometry were 5.72%, 13.57% , 19.39% respectively, and could induce the morphological and biochemical features of apoptosis in K562 cells. Conclusion: PLIP can significantly inhibit the growth and proliferation of human leukemia K562 cells in vitro. The mechanism may be related to the induction of apoptosis and the cell cycle arrest in K562 cells.