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研究了金龟子绿僵菌IMI330189的液体发酵动力学。利用Sigmoid函数构建了该菌株液体发酵过程中的菌体生长和底物消耗的动力学模型,并运用Origin7.5软件拟合求解出各模型参数。结果表明,模型能够较好地拟合绿僵菌IMI330189液体发酵过程,其比生长速率在发酵第22.8h达到最大值,为0.084h-1;总糖比消耗速率在第9.6h达到最大值,为0.246h-1;总氮比消耗速率在第10.3h达到最大值,为0.007h-1;菌体对总糖的得率系数在39.8h达到最高,为0.861g/g。模型拟合和实验数据具有良好的适应性,基本反映了绿僵菌IMI330189液体发酵过程的动力学特征,为其液体发酵工艺的优化和发展奠定了基础。
The liquid fermentation kinetics of Metarhizium anisopliae IMI330189 was studied. The kinetic model of bacterial growth and substrate consumption in liquid fermentation was constructed by using Sigmoid function and the parameters of each model were solved by using Origin7.5 software. The results showed that the model could well fit the Metarhizium anisopliae IMI330189 liquid fermentation process and its specific growth rate reached the maximum value of 0.084 h-1 at 22.8 h after fermentation. The total sugar consumption rate reached the maximum at 9.6 h, Was 0.246h-1. The total nitrogen consumption rate reached the maximum at 10.3h, which was 0.007h-1. The yield coefficient of total sugar reached the highest at 39.8h, which was 0.861g / g. The model fitting and experimental data have good adaptability and basically reflect the kinetic characteristics of liquid fermentation of Metarhizium anisopliae IMI330189, which lays the foundation for the optimization and development of the liquid fermentation process.